| Gene | Nsg1 |
| Chromosome | 5 |
| Deletion Start | 38,159,002 |
| Deletion End | 38,138,665 |
| Deletion Size | 20,338 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,158,840 | 38,158,858 | ATTCCCGCGCTGCTGAGTG |
| TUP | 38,158,807 | 38,158,834 | CCCTAAATCTTCGGCTGCAACTGAAACA |
| TUR | 38,158,786 | 38,158,803 | ACGCGCACAAGGCGTTTC |
| TDF | 38,139,485 | 38,139,506 | GTCCAAATGGAAGGCAGAACAG |
| TDP | 38,139,456 | 38,139,482 | AGTCAGTCATCTGGAGTTCTGGGTCCA |
| TDR | 38,139,435 | 38,139,454 | AAGCCACAGGGCAGGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCATCTCTCAAGCAGAGGG |
| SD | GGTCTGCAGATAGCAAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 468 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 469 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11298A-C4 | VGB6 | C57BL/6NTac | |
| 11298A-D11 | VGB6 | C57BL/6NTac | |
| 11298A-G10 | VGB6 | C57BL/6NTac | |
| 11298A-H1 | VGB6 | C57BL/6NTac | |
