| Gene | Lysmd4 |
| Chromosome | 7 |
| Deletion Start | 67,223,622 |
| Deletion End | 67,226,479 |
| Deletion Size | 2,858 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,224,066 | 67,224,088 | GCTCAGGAAGGGTCAGTTAGTTG |
| TUP | 67,224,091 | 67,224,116 | CACCTCAGTTCAGGGTGTGCTTGTCC |
| TUR | 67,224,140 | 67,224,160 | GCTCAGAGGCCACACTGTTTC |
| TDF | 67,225,486 | 67,225,503 | GAGCCAGCTTGGGCTTTG |
| TDP | 67,225,507 | 67,225,533 | AGCTTGTCTGGTCACTTGCTCAGCTGG |
| TDR | 67,225,559 | 67,225,579 | CTGTCACCCTTCCTGTCTAAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTGAGACACAGTGGTTTC |
| SD | AGTGCTGCACAGGAACTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 425 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 345 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11300A-A4 | VGB6 | C57BL/6NTac | |
| 11300A-A8 | VGB6 | C57BL/6NTac | |
| 11300A-C4 | VGB6 | C57BL/6NTac | |
| 11300A-D6 | VGB6 | C57BL/6NTac | |
| 11300A-D11 | VGB6 | C57BL/6NTac | |
| 11300A-E8 | VGB6 | C57BL/6NTac | |
