| Gene | Fastkd5 |
| Chromosome | 2 |
| Deletion Start | 130,616,530 |
| Deletion End | 130,614,221 |
| Deletion Size | 2,310 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,616,294 | 130,616,313 | CCCACCTGGGTTTGGAGTTC |
| TUP | 130,616,263 | 130,616,291 | CAAAGCTTCTACCCTTAATGCCAGCACAC |
| TUR | 130,616,239 | 130,616,260 | CAGCACTGGATGAGTCTGGATG |
| TDF | 130,615,120 | 130,615,140 | ACCAGTACCCAGAGCACCTTC |
| TDP | 130,615,097 | 130,615,118 | CACCTGCCTGATTGGCTTGGCA |
| TDR | 130,615,048 | 130,615,070 | CTGGGCTCAGAGCAACATTTATG |
PCR Primers
| Primer | Sequence |
| SU | CACCTGGCTGCACCTAATTC |
| SD | TACTTGGAGGACAGGTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 320 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11301A-C1 | VGB6 | C57BL/6NTac | |
| 11301A-F7 | VGB6 | C57BL/6NTac | |
| 11301A-D12 | VGB6 | C57BL/6NTac | |
| 11301B-B1 | VGB6 | C57BL/6NTac | |
| 11301B-B6 | VGB6 | C57BL/6NTac | |
| 11301B-C11 | VGB6 | C57BL/6NTac | |
| 11301B-B10 | VGB6 | C57BL/6NTac | |
| 11301B-F2 | VGB6 | C57BL/6NTac | |
| 11301B-D4 | VGB6 | C57BL/6NTac | |
| 11301B-E1 | VGB6 | C57BL/6NTac | |
| 11301B-E5 | VGB6 | C57BL/6NTac | |
