| Gene | Trpd52l3 |
| Chromosome | 19 |
| Deletion Start | 30,003,831 |
| Deletion End | 30,004,547 |
| Deletion Size | 717 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,004,056 | 30,004,075 | CCGCCTAGCTTCAGAACCAG |
| TUP | 30,004,076 | 30,004,101 | ATCTGACGGAGGCTGAGCAACTGGAA |
| TUR | 30,004,143 | 30,004,161 | TGCAAGCAGGTCACGTAAG |
| TDF | 30,004,457 | 30,004,476 | GTGATCCACACTCGGCTCTG |
| TDP | 30,004,482 | 30,004,511 | TGGATATGAAACTGTTGCAGGGCTTGATGA |
| TDR | 30,004,525 | 30,004,545 | GGGTTACTCTGGCTTCAGGAC |
PCR Primers
| Primer | Sequence |
| SU | GCCTATGTTCCAAGTGCTAG |
| SD | CTTTAGGATCTGGTCTAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 394 bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 399 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11304B-A6 | VGB6 | C57BL/6NTac | |
| 11304B-G2 | VGB6 | C57BL/6NTac | |
| 11304C-C1 | VGB6 | C57BL/6NTac | |
| 11304C-G1 | VGB6 | C57BL/6NTac | |
| 11304C-G8 | VGB6 | C57BL/6NTac | |
