| Gene | Svs1 |
| Chromosome | 6 |
| Deletion Start | 48,987,063 |
| Deletion End | 48,991,614 |
| Deletion Size | 4,552 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,987,241 | 48,987,258 | TGCAGCCATCCACGTTAC |
| TUP | 48,987,265 | 48,987,288 | TGGCCAAGAACTCCGTGTTCCTCA |
| TUR | 48,987,311 | 48,987,330 | GGAAAGCCAGCACCTCATAC |
| TDF | 48,991,088 | 48,991,111 | ACCAGACACCAGACTCCCTAGAAG |
| TDP | 48,991,116 | 48,991,142 | ACTCAATGCCTGCACAGTTCAGCTACA |
| TDR | 48,991,152 | 48,991,171 | AGCTGGGCCAGACACAAGAC |
PCR Primers
| Primer | Sequence |
| SU | TCCGCTGCTGTGACTCATG |
| SD | TGAATGTAATACATACTAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 424 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11307A-C7 | VGB6 | C57BL/6NTac | |
| 11307A-A12 | VGB6 | C57BL/6NTac | |
| 11307A-G1 | VGB6 | C57BL/6NTac | |
