| Gene | Gpr77 |
| Chromosome | 7 |
| Deletion Start | 16,237,955 |
| Deletion End | 16,236,904 |
| Deletion Size | 1,052 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,237,591 | 16,237,611 | GTCCTCCATCACCATTCTGTC |
| TUP | 16,237,565 | 16,237,589 | ATGTATGCCAGTGTGCTGCTCCTGA |
| TUR | 16,237,522 | 16,237,541 | GAGGGCCTGAAAGCCAGTAG |
| TDF | 16,237,172 | 16,237,193 | CACGTCCTGCGGGTAATCATTG |
| TDP | 16,237,147 | 16,237,168 | CGGCTCCTCCGCACTCCTTGCT |
| TDR | 16,237,099 | 16,237,120 | AGGGCCAGGCCATTAAACAGAG |
PCR Primers
| Primer | Sequence |
| SU | GCAATAACCGGCACAGAATG |
| SD | ATCTCAACCTTGACATAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 281 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11308A-C5 | VGB6 | C57BL/6NTac | |
| 11308A-C8 | VGB6 | C57BL/6NTac | |
| 11308A-F1 | VGB6 | C57BL/6NTac | |
