| Gene | Taar7a |
| Chromosome | 10 |
| Deletion Start | 23,993,478 |
| Deletion End | 23,992,386 |
| Deletion Size | 1,093 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,993,197 | 23,993,217 | CTGGCCTGTGCTGATTTCTTA |
| TUP | 23,993,172 | 23,993,195 | TGGGTCTGACTGTGATGCCCTTCA |
| TUR | 23,993,098 | 23,993,125 | CCCTTCAAAACAAGAATGGAATTTACAA |
| TDF | 23,992,889 | 23,992,910 | GGCTGGAGGATCTAGTGAGTGT |
| TDP | 23,992,866 | 23,992,888 | CCTCACCTGTGTGGGTGGCTGTC |
| TDR | 23,992,783 | 23,992,807 | AGAGTAGACAGTCATCATCACAAGA |
PCR Primers
| Primer | Sequence |
| SU | CCTGGGTCTGTAAAGTTGGG |
| SD | GGGATTGAGCTAATATGAAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 128 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11310A-D5 | VGB6 | C57BL/6NTac | |
| 11310A-D12 | VGB6 | C57BL/6NTac | |
| 11310A-F3 | VGB6 | C57BL/6NTac | |
| 11310A-F5 | VGB6 | C57BL/6NTac | |
| 11310A-H8 | VGB6 | C57BL/6NTac | |
