| Gene | Tchhl1 |
| Chromosome | 3 |
| Deletion Start | 93,469,480 |
| Deletion End | 93,471,919 |
| Deletion Size | 2,440 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,470,412 | 93,470,434 | AGCCCAGCAATGAAGAAGGTGAG |
| TUP | 93,470,436 | 93,470,462 | TAGGTGATGAGCCTATGGTGAGCCCAT |
| TUR | 93,470,468 | 93,470,488 | CAGGCTGTGGGTCTTAATGTC |
| TDF | 93,470,945 | 93,470,966 | GAGCCCAAAGCTGATGCTAAAG |
| TDP | 93,470,970 | 93,470,992 | CTGAGAGCCACGGATTGCCAGCA |
| TDR | 93,471,000 | 93,471,021 | TGGTCCCTTGTATTGTGTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CTAGACTGACTCAAGGTGAG |
| SD | AGAATGCCAAGAGAAGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 343 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11311A-G3 | VGB6 | C57BL/6NTac | |
| 11311B-D12 | VGB6 | C57BL/6NTac | |
| 11311B-H6 | VGB6 | C57BL/6NTac | |
