| Gene | Ccdc67 |
| Chromosome | 9 |
| Deletion Start | 15,626,679 |
| Deletion End | 15,599,702 |
| Deletion Size | 26,978 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 15,626,442 | 15,626,463 | GACAGCACTTCCTGACTGATAC |
| TUP | 15,626,413 | 15,626,438 | TGGCGTTATAGATTCGGAACCCATGC |
| TUR | 15,626,376 | 15,626,394 | GCGCTGTGTTGTGGACTTG |
| TDF | 15,599,815 | 15,599,836 | TGTGAAGCCACTCAAAGAGATG |
| TDP | 15,599,782 | 15,599,811 | TCATTATCGAGAAGTTAAAGTCGGCCGTGA |
| TDR | 15,599,762 | 15,599,779 | CCTGCTCAGCGCGATTTC |
PCR Primers
| Primer | Sequence |
| SU | CAAAGGCAAAGGATAGGCAC |
| SD | GGGTGGTGACTGAATACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 503 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 396 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11314A-D1 | VGB6 | C57BL/6NTac | |
| 11314B-A6 | VGB6 | C57BL/6NTac | |
