| Gene | Taar7b |
| Chromosome | 10 |
| Deletion Start | 23,999,942 |
| Deletion End | 24,001,179 |
| Deletion Size | 1,238 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,000,153 | 24,000,177 | TTCTTCATTTCAGGCAGCTACACTC |
| TUP | 24,000,178 | 24,000,201 | CCCTGCCAACTTCCTGGTGGTGTC |
| TUR | 24,000,220 | 24,000,242 | GCATCACAGTCAATCCCACCAAG |
| TDF | 24,000,948 | 24,000,971 | TCAAACTCATTGTCTCTGGCAAAG |
| TDP | 23,992,420 | 23,992,445 | TGAGGGAGAATTCCTCAACCACCAAC |
| TDR | 24,001,036 | 24,001,056 | TCCTGCTTTGAGTCTCTCCAG |
PCR Primers
| Primer | Sequence |
| SU | TCACTTGCCTCACCGTACAC |
| SD | GCCAACAATCAGATGTTTAGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11316A-A2 | VGB6 | C57BL/6NTac | |
| 11316A-F8 | VGB6 | C57BL/6NTac | |
| 11316A-G11 | VGB6 | C57BL/6NTac | |
| 11316A-C9 | VGB6 | C57BL/6NTac | |
| 11316A-H8 | VGB6 | C57BL/6NTac | |
