| Gene | Cxcr7 |
| Chromosome | 1 |
| Deletion Start | 90,213,805 |
| Deletion End | 90,214,909 |
| Deletion Size | 1,105 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,214,051 | 90,214,069 | CGACACGCACTGCTACATC |
| TUP | 90,214,070 | 90,214,093 | TTGAACCTGGCCATTGCAGACCTG |
| TUR | 90,214,123 | 90,214,140 | TGCTGCACGAGACTGACC |
| TDF | 90,214,522 | 90,214,541 | GCTCGCTAGAGCCATGTCAG |
| TDP | 90,214,542 | 90,214,565 | CATCAGGCGACCAGGAGAAGCACA |
| TDR | 90,214,598 | 90,214,619 | GCAGCCAACATACCAGGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | CTAGGACACAGCCAAGCTG |
| SD | ATGGGCATCCAGGCGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 396 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11317A-A3 | VGB6 | C57BL/6NTac | |
| 11317A-C1 | VGB6 | C57BL/6NTac | |
| 11317A-D4 | VGB6 | C57BL/6NTac | |
| 11317A-G6 | VGB6 | C57BL/6NTac | |
| 11317A-C11 | VGB6 | C57BL/6NTac | |
