| Gene | Sowahb |
| Chromosome | 5 |
| Deletion Start | 93,044,855 |
| Deletion End | 93,042,472 |
| Deletion Size | 2,384 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,044,776 | 93,044,795 | CGTAACGCAGAGCTGCTGAG |
| TUP | 93,044,752 | 93,044,775 | CCACTTCAAGAGCTTCCTGCGCGA |
| TUR | 93,044,682 | 93,044,700 | GGCCACCGAGTTCACGAAG |
| TDF | 93,042,714 | 93,042,732 | CCCAGCCAGGAAGGTCAAG |
| TDP | 93,042,686 | 93,042,713 | AGCCGGGAAGTGTCTAGAAATGTCACCC |
| TDR | 93,042,662 | 93,042,681 | GCAAAGCAGCCAAGGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGGCCAGCGTCGTTCAGG |
| SD | AGAAAATACTGTGCCTTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 386 bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 314 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11322D-C11 | VGB6 | C57BL/6NTac | |
| 11322D-E9 | VGB6 | C57BL/6NTac | |
| 11322E-B1 | VGB6 | C57BL/6NTac | |
