| Gene | Zfp238 |
| Chromosome | 1 |
| Deletion Start | 177,447,086 |
| Deletion End | 177,448,760 |
| Deletion Size | 1,675 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 177,447,163 | 177,447,184 | GGCTTTCTTTGTGACTGCACTG |
| TUP | 177,447,185 | 177,447,208 | TTCTGGTGGGAGATGCCCAGTTCC |
| TUR | 177,447,221 | 177,447,241 | TGCTGCATGAAGCCAGAACAG |
| TDF | 177,448,004 | 177,448,026 | TGAGCACTAACAACAGGGTACAG |
| TDP | 177,448,027 | 177,448,046 | TATGAGCCAGCCCACCTGGC |
| TDR | 177,448,075 | 177,448,095 | TTTGTCCTCCCGATCCAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TAAGTCATAAGCTAAAGGAAGC |
| SD | GCCATGTGAAAGCGTTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 360 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 293 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11323A-B5 | VGB6 | C57BL/6NTac | |
| 11323A-E8 | VGB6 | C57BL/6NTac | |
| 11323A-H12 | VGB6 | C57BL/6NTac | |
