| Gene | Hoxc5 |
| Chromosome | 15 |
| Deletion Start | 26,126,768 |
| Deletion End | 26,132,200 |
| Deletion Size | 5,433 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,014,139 | 103,014,159 | AACATGCAAACTTGTGGGAAC |
| TUP | 103,014,160 | 103,014,182 | TATGGATCGGCCTCAGAGGTGCA |
| TUR | 103,014,219 | 103,014,238 | CAGGCGGTGGGAAAGTGATG |
| TDF | 103,015,241 | 103,015,259 | GCGGTCCCGAACCAGTTAC |
| TDP | 103,015,261 | 103,015,283 | CGCGCTACCAGACCCTGGAACTC |
| TDR | 103,015,334 | 103,015,355 | GAGACACAAGTTGTTGGCAATC |
PCR Primers
| Primer | Sequence |
| SU | ACATATCAAGATGCGTTTCGC |
| SD | ACTCAGGCTAGCTGAACTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 76887190 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | 76883657 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11324A-C1 | VGB6 | C57BL/6NTac | |
| 11324A-C3 | VGB6 | C57BL/6NTac | |
| 11324A-F10 | VGB6 | C57BL/6NTac | |
