| Gene | Tssk2 |
| Chromosome | 16 |
| Deletion Start | 17,898,738 |
| Deletion End | 17,899,877 |
| Deletion Size | 1,140 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,898,749 | 17,898,773 | GGTCCTAAGGAAGAAGGGTTACATC |
| TUP | 17,898,779 | 17,898,805 | AATCAATCTGGGCAAGGGCTCCTACGC |
| TUR | 17,898,834 | 17,898,855 | TGACTGCCACATTGAACTTGAG |
| TDF | 17,899,440 | 17,899,459 | CCACGCTCCAAGAACCTAAC |
| TDP | 17,899,460 | 17,899,486 | TGGTGAGTGCAAGGACCTCATCTACCG |
| TDR | 17,899,498 | 17,899,516 | AGCCGCCGGTTGACATCTG |
PCR Primers
| Primer | Sequence |
| SU | GCAGGGACTTGGCTCTAATG |
| SD | TGCCTCTCTGAAGTGAATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11326A-D4 | VGB6 | C57BL/6NTac | |
| 11326A-H4 | VGB6 | C57BL/6NTac | |
| 11326A-G3 | VGB6 | C57BL/6NTac | |
| 11326A-D10 | VGB6 | C57BL/6NTac | |
| 11326A-D6 | VGB6 | C57BL/6NTac | |
