| Gene | Pllp |
| Chromosome | 8 |
| Deletion Start | 94,696,167 |
| Deletion End | 94,676,122 |
| Deletion Size | 20,046 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,695,764 | 94,695,782 | ACCGTGCCCTGGCTTTCTC |
| TUP | 94,695,744 | 94,695,763 | CGCCGGGCAAAGTCCACTGC |
| TUR | 94,695,700 | 94,695,720 | ACAGAGCGGTCGCATTTAGTG |
| TDF | 94,676,848 | 94,676,867 | CCAGGACAGGACAGCCAATG |
| TDP | 94,676,822 | 94,676,847 | CGTGGTGTCTTCCATCTTCCTGGTGA |
| TDR | 94,676,763 | 94,676,782 | TGCTTTCAGAGGCTTGAGTC |
PCR Primers
| Primer | Sequence |
| SU | GTAGATCGGTCTACGGACTTC |
| SD | GCTGAGGGTCTGTTAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11327A-G12 | VGB6 | C57BL/6NTac | |
| 11327B-B4 | VGB6 | C57BL/6NTac | |
| 11327B-C3 | VGB6 | C57BL/6NTac | |
| 11327B-D11 | VGB6 | C57BL/6NTac | |
