| Gene | Siglech |
| Chromosome | 7 |
| Deletion Start | 55,768,271 |
| Deletion End | 55,772,576 |
| Deletion Size | 4,306 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,768,901 | 55,768,921 | CCCAACATCAGCATCCCACAG |
| TUP | 55,768,923 | 55,768,946 | CACTGGAGCTTGGTCGTCCTACCA |
| TUR | 55,768,949 | 55,768,968 | CAGGGCACAGAGCACATCAC |
| TDF | 55,772,426 | 55,772,447 | GGAGTTCAGGACAAGAAGAGAC |
| TDP | 55,772,450 | 55,772,474 | CTACACCTGCTCCCTTTCATGCCCA |
| TDR | 55,772,475 | 55,772,495 | GAGGAGACAGAGCCTTCAATG |
PCR Primers
| Primer | Sequence |
| SU | CTCTGAACCACACCCACAC |
| SD | GGGATGATCAAGATTGGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 340 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 346 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11330A-B1 | VGB6 | C57BL/6NTac | |
| 11330A-G3 | VGB6 | C57BL/6NTac | |
| 11330A-B7 | VGB6 | C57BL/6NTac | |
