| Gene | Ppm1l |
| Chromosome | 3 |
| Deletion Start | 69,317,570 |
| Deletion End | 69,342,875 |
| Deletion Size | 25,306 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,317,726 | 69,317,746 | GACGCGGTGAAGATGGTGAAG |
| TUP | 69,317,749 | 69,317,775 | CAAGGTGGCCGAGATTATGCAGAACGA |
| TUR | 69,317,787 | 69,317,806 | GGCCTCCAGCACATCAAGTC |
| TDF | 69,342,271 | 69,342,289 | GGTCGTGAGGCACAGGTTG |
| TDP | 69,342,290 | 69,342,316 | AGAACCACTGAGAGCACAGACTTCGGA |
| TDR | 69,342,319 | 69,342,339 | CCCTCCCTCGTGTCTTCTTAC |
PCR Primers
| Primer | Sequence |
| SU | CCAGACAAAAGGGAAACTGC |
| SD | AGACTGTAAAGATGGCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11335A-A6 | VGB6 | C57BL/6NTac | |
| 11335A-C5 | VGB6 | C57BL/6NTac | |
