Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

11338 Sowahd Status: ES cell colonies screened / QC positives

Gene	Sowahd
Chromosome	X
Deletion Start	37,048,909
Deletion End	37,049,907
Deletion Size	999
Genome Build	38

MGI ID	MGI:3045274
NCBI Gene ID	245381
BAC ID	311M4
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	37,049,020	37,049,041	CATTGCCTTGGCAGGTACAAAG
TUP	37,049,042	37,049,064	TACAAGCCGTCAGGGACAGTGCC
TUR	37,049,081	37,049,100	CAGGGCGCTGTGGAAGATTC
TDF	37,049,578	37,049,599	CGTCGAGATCACAGTGGCAATC
TDP	37,049,602	37,049,624	CCTTGTCACTATCTGCGGCCCGA
TDR	37,049,659	37,049,679	GCGTTCAATCTCCCACTCTTC

PCR Primers

Primer	Sequence
SU	CTGGTCCCCAGAATGTAGAC
SD	CTTCTCTTCCAAGGAGGATC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	388 bp	-	+	+
TUF	TUR	81 bp	+	+	-
TDF	TDR	102 bp	+	+	-
NeoFwd	SD	331 bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
11338A-B1	VGB6	C57BL/6NTac
11338B-B2	VGB6	C57BL/6NTac
11338B-C8	VGB6	C57BL/6NTac
11338B-F4	VGB6	C57BL/6NTac