| Gene | Prss39 |
| Chromosome | 1 |
| Deletion Start | 34,498,443 |
| Deletion End | 34,502,948 |
| Deletion Size | 4,506 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,498,507 | 34,498,526 | CCTGCTGGCTGCTTTCTTAC |
| TUP | 34,498,529 | 34,498,555 | TGTTTCAGCCTCTTACATGCTCAGGAC |
| TUR | 34,498,582 | 34,498,601 | TGAGCGAGGTGTTCGATGTG |
| TDF | 34,502,644 | 34,502,665 | GGTCTGCTTCCTGAATAGCTTC |
| TDP | 34,502,669 | 34,502,690 | TACGTGGTAGGGCTGGCCAACT |
| TDR | 34,502,697 | 34,502,718 | TGGATCGGTTCAAGACATGCTC |
PCR Primers
| Primer | Sequence |
| SU | ATGTGGTGAGTGCTTTTGCC |
| SD | TTAGCAGGTCAGCTCCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 365 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 310 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11341B-E1 | VGB6 | C57BL/6NTac | |
| 11341A-A10 | VGB6 | C57BL/6NTac | |
