| Gene | Znrf4 |
| Chromosome | 17 |
| Deletion Start | 56,512,302 |
| Deletion End | 56,511,232 |
| Deletion Size | 1,071 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,512,253 | 56,512,271 | TGGCCCTGGTGACTTTCTG |
| TUP | 56,512,228 | 56,512,247 | TCCTGTCCCTGTCGCCCACG |
| TUR | 56,512,139 | 56,512,156 | TATCCGCGTGCCCTCTTG |
| TDF | 56,511,709 | 56,511,728 | CATGCCAGAAGGCACAGGTG |
| TDP | 56,511,690 | 56,511,708 | CGCACGTTCACGCGCCTCA |
| TDR | 56,511,657 | 56,511,676 | CGTAGTCGTCCAGGCAGATG |
PCR Primers
| Primer | Sequence |
| SU | GGTCGGAAGCGTCCAGAAC |
| SD | AGACTGGACAAACACTGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 133 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 344 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11350B-B12 | VGB6 | C57BL/6NTac | |
| 11350B-C4 | VGB6 | C57BL/6NTac | |
| 11350B-E7 | VGB6 | C57BL/6NTac | |
| 11350B-G11 | VGB6 | C57BL/6NTac | |
| 11350B-H5 | VGB6 | C57BL/6NTac | |
| 11350C-E8 | VGB6 | C57BL/6NTac | |
