11352   Foxd4     Status: ES cell colonies screened / QC positives

GeneFoxd4
Chromosome19
Deletion Start24,900,831
Deletion End24,899,446
Deletion Size1,386
Genome Build38
MGI IDMGI:1347467
NCBI Gene ID14237
BAC ID169B23
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 19

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF24,900,79524,900,814GCACCTTCGTTCCACTCCAC
TUP24,900,76724,900,792CCCAGTCCTCTAAGTTCCGACCAGGA
TUR24,900,71624,900,738TCCGTCTGATCTCCATCTTCCTC
TDF24,899,65624,899,674GCAGCAAGAACAGCATCAC
TDP24,899,63524,899,655TGTGCCATTAGCTGCGCTCCC
TDR24,899,53324,899,551AAGAGCGGCCAGGGTTGTC

PCR Primers

PrimerSequence
SUCGAGGAACGGCAAAGAAGG
SDAGCGGACAAGACCAAGCAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev347 bp-++
TUFTUR99 bp++-
TDFTDR142 bp++-
NeoFwdSD280 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11352A-B8VGB6C57BL/6NTac
11352A-C5VGB6C57BL/6NTac
11352A-C9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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