| Gene | Lrrc10 |
| Chromosome | 10 |
| Deletion Start | 117,045,426 |
| Deletion End | 117,046,251 |
| Deletion Size | 845 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,045,751 | 117,045,770 | TCTGTGACCTCCCGGATGAG |
| TUP | 117,045,773 | 117,045,795 | CAGCCTGCTCCAGAACCTACGGA |
| TUR | 117,045,807 | 117,045,826 | CGGGTGAGGCAGTTGGATTC |
| TDF | 117,046,149 | 117,046,167 | GAGACACCAGAGCCTGACC |
| TDP | 117,046,173 | 117,046,196 | AAAGCTAGGCGGTACGCACTGGCC |
| TDR | 117,046,226 | 117,046,247 | TCAAGAGCTGGAAGGAAGAAGG |
PCR Primers
| Primer | Sequence |
| SU | GGAGAGAGTAGATAAGAGATC |
| SD | TAGCTCGTGTGAAGGAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 399 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11358A-C11 | VGB6 | C57BL/6NTac | |
| 11358A-D12 | VGB6 | C57BL/6NTac | |
| 11358A-E4 | VGB6 | C57BL/6NTac | |
| 11358A-F4 | VGB6 | C57BL/6NTac | |
| 11358A-H7 | VGB6 | C57BL/6NTac | |
| 11358A-H12 | VGB6 | C57BL/6NTac | |
