| Gene | 4930430A15Rik |
| Chromosome | 2 |
| Deletion Start | 111,229,409 |
| Deletion End | 111,192,962 |
| Deletion Size | 36,448 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,229,127 | 111,229,146 | TGGTCTGTGTGGAGGATGTG |
| TUP | 111,229,099 | 111,229,126 | AGACAGCCTCTGTGATATCCGAAGACCA |
| TUR | 111,229,045 | 111,229,065 | TCCACGGGAGAAGGAGCATAG |
| TDF | 111,193,238 | 111,193,261 | AAGGCACTACAAGTGAGCAGTATC |
| TDP | 111,193,203 | 111,193,231 | AATCCTCTATGCTAATCTGGCTACTGCCC |
| TDR | 111,193,177 | 111,193,200 | GTCTTGATTTCCTCCCAGTCAATG |
PCR Primers
| Primer | Sequence |
| SU | AAGCCTTGCTGTTGTGCATG |
| SD | GAAGAGGACAGGAAGTAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 363 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 255 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11364B-D12 | VGB6 | C57BL/6NTac | |
