| Gene | Zbtb22 |
| Chromosome | 17 |
| Deletion Start | 33,916,867 |
| Deletion End | 33,918,818 |
| Deletion Size | 1,952 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,917,000 | 33,917,019 | ACCAGTGCCCTCCTGGAATC |
| TUP | 33,917,022 | 33,917,040 | TCAACCAGCAGCGGCTCCA |
| TUR | 33,917,052 | 33,917,071 | CACGCGGATGGACACATCAC |
| TDF | 33,918,508 | 33,918,528 | CTGCGCCAAGAAGTTCATGTG |
| TDP | 33,918,530 | 33,918,553 | CGGGACAGTTTCATGCGTCACCGG |
| TDR | 33,918,633 | 33,918,650 | GCAATGCTGGCCCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCGGTTTGGTGGTGTTTG |
| SD | TCTGTGACTCTAGGATGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 354 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 143 bp | + | + | - |
| NeoFwd | SD | 345 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11365A-A8 | VGB6 | C57BL/6NTac | |
| 11365A-G8 | VGB6 | C57BL/6NTac | |
| 11365A-D3 | VGB6 | C57BL/6NTac | |
| 11365A-E6 | VGB6 | C57BL/6NTac | |
| 11365A-G2 | VGB6 | C57BL/6NTac | |
| 11365A-C2 | VGB6 | C57BL/6NTac | |
