| Gene | S100g |
| Chromosome | X |
| Deletion Start | 162,964,193 |
| Deletion End | 162,962,133 |
| Deletion Size | 2,061 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 162,963,284 | 162,963,305 | GGGTTGGAAAGGTTTGGTTCAC |
| TUP | 162,963,260 | 162,963,280 | AGGCACAGGGCTGCTGACGCC |
| TUR | 162,963,218 | 162,963,236 | GAAGAGGGCGTTGGATCTC |
| TDF | 162,962,739 | 162,962,759 | CGGATTCCGATCCTAGCACTC |
| TDP | 162,962,709 | 162,962,735 | TCAGGTAACTCACAACCACCTGGAGCT |
| TDR | 162,962,671 | 162,962,692 | TGCTCTCAGAGGCCATTAACTG |
PCR Primers
| Primer | Sequence |
| SU | TAAGCAAGCCTCCTCCTATG |
| SD | TGCATGTACTTGCTCAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 387 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 377 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11367A-D9 | VGB6 | C57BL/6NTac | |
| 11367A-F10 | VGB6 | C57BL/6NTac | |
| 11367A-F11 | VGB6 | C57BL/6NTac | |
| 11367A-G1 | VGB6 | C57BL/6NTac | |
| 11367A-G5 | VGB6 | C57BL/6NTac | |
| 11367A-H1 | VGB6 | C57BL/6NTac | |
