| Gene | Rptn |
| Chromosome | 3 |
| Deletion Start | 93,394,982 |
| Deletion End | 93,398,723 |
| Deletion Size | 3,742 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,395,707 | 93,395,729 | GAAAGCACAGGCAGACAACATAG |
| TUP | 93,395,732 | 93,395,759 | AGAGGTATGAAGGAGAACGCCGGAACTC |
| TUR | 93,395,761 | 93,395,783 | TGTCCTTCAGACTGATTGTGGTG |
| TDF | 93,398,302 | 93,398,326 | GAGGAACAAAGCCATCAAACAAGTG |
| TDP | 93,398,329 | 93,398,353 | AGGCAAACCCATGTAGACGAGCAGA |
| TDR | 93,398,356 | 93,398,375 | TGCCTGTGTTGTCTCTGCTG |
PCR Primers
| Primer | Sequence |
| SU | CTTCTGAATCACTATCATGATAG |
| SD | CTAGCGATGATTCAAATCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 392 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 364 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11369A-B9 | VGB6 | C57BL/6NTac | |
| 11369A-B3 | VGB6 | C57BL/6NTac | |
| 11369B-B7 | VGB6 | C57BL/6NTac | |
| 11369B-D1 | VGB6 | C57BL/6NTac | |
| 11369B-D8 | VGB6 | C57BL/6NTac | |
| 11369B-H9 | VGB6 | C57BL/6NTac | |
