| Gene | Gpr20 |
| Chromosome | 15 |
| Deletion Start | 73,696,535 |
| Deletion End | 73,695,439 |
| Deletion Size | 1,097 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,696,146 | 73,696,166 | GTCCTGGGCTACTTCCTCAAC |
| TUP | 73,696,119 | 73,696,144 | TGCACTGTTCCATCCTCTTCCTCACC |
| TUR | 73,696,099 | 73,696,116 | TAGCGGTCCACGCAGATG |
| TDF | 73,695,522 | 73,695,540 | GGCCTCAGCTCCCATTCAC |
| TDP | 73,695,498 | 73,695,520 | TCCTCAGCATCGGTTCCCACACC |
| TDR | 73,695,476 | 73,695,497 | CGTTGGTCAAAGGCTGGGTAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTCATAGCAGCCCTGTGTG |
| SD | ACTACACAGCCACAGGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11371A-A6 | VGB6 | C57BL/6NTac | |
| 11371A-B10 | VGB6 | C57BL/6NTac | |
| 11371A-D4 | VGB6 | C57BL/6NTac | |
| 11371A-E8 | VGB6 | C57BL/6NTac | |
