| Gene | Lrrn2 |
| Chromosome | 1 |
| Deletion Start | 132,937,202 |
| Deletion End | 132,939,411 |
| Deletion Size | 2,210 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,937,677 | 132,937,696 | CCTTTGCAGGGCTGGGTAAC |
| TUP | 132,937,697 | 132,937,726 | CTGCTGAGACTGCACCTCAATTCCAACCTG |
| TUR | 132,937,727 | 132,937,748 | AGCGGCTGTCGATGGTCCTAAG |
| TDF | 132,938,943 | 132,938,963 | ACAACATTACCCGCCTCCTTC |
| TDP | 132,938,964 | 132,938,984 | CGGCCACAGAGTACTGGGCCT |
| TDR | 132,939,017 | 132,939,036 | GCCCAAACACAAGCCAACTG |
PCR Primers
| Primer | Sequence |
| SU | TCCAGTGCACAAAGATGAGG |
| SD | TTCCTGTTCCTTGGACACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 306 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 275 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11374A-B8 | VGB6 | C57BL/6NTac | |
| 11374A-C3 | VGB6 | C57BL/6NTac | |
| 11374A-F3 | VGB6 | C57BL/6NTac | |
| 11374A-F1 | VGB6 | C57BL/6NTac | |
| 11374A-E11 | VGB6 | C57BL/6NTac | |
| 11374A-H10 | VGB6 | C57BL/6NTac | |
