| Gene | Mamdc4 |
| Chromosome | 2 |
| Deletion Start | 25,571,172 |
| Deletion End | 25,563,287 |
| Deletion Size | 7,886 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,570,874 | 25,570,895 | CTCTGTGTAGTCCTCGATGTCC |
| TUP | 25,570,842 | 25,570,869 | ACTAGCTCTGTAGACCAGGCTGGACTCA |
| TUR | 25,570,816 | 25,570,833 | GGTAGAGGCAGGCAGTTC |
| TDF | 25,563,419 | 25,563,440 | CCATGTCCCATCCTGTCAGAAC |
| TDP | 25,563,393 | 25,563,418 | CACACTGCCTTTCAACAGCAGCTGTC |
| TDR | 25,563,331 | 25,563,351 | TGGGTTGCTGGTGATTGATTC |
PCR Primers
| Primer | Sequence |
| SU | CTAGGAATACTTTTACAGCTAG |
| SD | CAGCCTGAATATCTGGGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 354 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 287 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11380A-E4 | VGB6 | C57BL/6NTac | |
| 11380B-H9 | VGB6 | C57BL/6NTac | |
| 11380B-B4 | VGB6 | C57BL/6NTac | |
| 11380B-B9 | VGB6 | C57BL/6NTac | |
| 11380B-E5 | VGB6 | C57BL/6NTac | |
| 11380B-D5 | VGB6 | C57BL/6NTac | |
| 11380B-C6 | VGB6 | C57BL/6NTac | |
