| Gene | Sstr4 |
| Chromosome | 2 |
| Deletion Start | 148,395,457 |
| Deletion End | 148,396,667 |
| Deletion Size | 1,211 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,395,699 | 148,395,720 | ACAGCCACCAACATCTACCTAC |
| TUP | 148,395,722 | 148,395,743 | CAACCTGGCCGTCGCCGATGAG |
| TUR | 148,395,746 | 148,395,766 | AATGGCACGCTGAGCATGAAG |
| TDF | 148,396,409 | 148,396,427 | CAACTTCCGGCGCTCTTTC |
| TDP | 148,396,435 | 148,396,456 | TTCTGTGCCTGCGCTGCTGTCT |
| TDR | 148,396,468 | 148,396,487 | CTCTTCCTCAGCACCTCCAG |
PCR Primers
| Primer | Sequence |
| SU | ACATGGCAGAGGTGGCGC |
| SD | AGGAATGGATGGTACTTTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 410 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 278 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11381A-H4 | VGB6 | C57BL/6NTac | |
| 11381A-H7 | VGB6 | C57BL/6NTac | |
| 11381B-D4 | VGB6 | C57BL/6NTac | |
| 11381B-F4 | VGB6 | C57BL/6NTac | |
