| Gene | Rrad |
| Chromosome | 8 |
| Deletion Start | 104,630,910 |
| Deletion End | 104,628,421 |
| Deletion Size | 2,490 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,630,143 | 104,630,162 | TGACCACACCCACCCTTGAC |
| TUP | 104,630,117 | 104,630,141 | CAGGCAGCAGGATCCCTAGACACCC |
| TUR | 104,630,074 | 104,630,095 | GGACACGACCTCCATCTCTTTG |
| TDF | 104,628,874 | 104,628,896 | CCAGCAATGTTTAGGGTTCTCAG |
| TDP | 104,628,843 | 104,628,872 | ACAGGAACTAGGATCTCCATGTCTCTAGGA |
| TDR | 104,628,789 | 104,628,809 | GCCATATCCAGCAGCTACAAC |
PCR Primers
| Primer | Sequence |
| SU | GGGTCTGAGTCTGAACAGGG |
| SD | TGAGCAGGAGGGACCTTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 428 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 557 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11383A-A8 | VGB6 | C57BL/6NTac | |
| 11383A-G9 | VGB6 | C57BL/6NTac | |
| 11383A-G6 | VGB6 | C57BL/6NTac | |
| 11383A-F1 | VGB6 | C57BL/6NTac | |
| 11383A-C7 | VGB6 | C57BL/6NTac | |
| 11383A-B12 | VGB6 | C57BL/6NTac | |
