| Gene | Taar2 |
| Chromosome | 10 |
| Deletion Start | 23,938,575 |
| Deletion End | 23,941,643 |
| Deletion Size | 3,069 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,939,452 | 23,939,473 | TCAACATGGATGGAGGGACTTC |
| TUP | 23,939,475 | 23,939,496 | TGGGCCCTAGTTGATGGCCCTG |
| TUR | 23,939,515 | 23,939,534 | TCTGAACCCACATGGCACTG |
| TDF | 23,940,788 | 23,940,809 | CACCGACTTCCTTCTCGGATTC |
| TDP | 23,940,815 | 23,940,844 | CATGCCATATAGTATGGTCAGGTCAGTGGA |
| TDR | 23,940,848 | 23,940,869 | CGTAAGTCCGAAATACCAGCAG |
PCR Primers
| Primer | Sequence |
| SU | ATCAGAATTGAGAGCCTCCG |
| SD | AAATCATCTCTCTCACTTCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11384A-A5 | VGB6 | C57BL/6NTac | |
| 11384A-E5 | VGB6 | C57BL/6NTac | |
| 11384A-G12 | VGB6 | C57BL/6NTac | |
| 11384A-F6 | VGB6 | C57BL/6NTac | |
