| Gene | Lrrc49 |
| Chromosome | 9 |
| Deletion Start | 60,687,856 |
| Deletion End | 60,666,294 |
| Deletion Size | 21,563 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,687,478 | 60,687,499 | TGTTCTTAGACACTCCCACCTC |
| TUP | 60,687,447 | 60,687,473 | ATTCAAGCACTGACATCCTGACTGGCA |
| TUR | 60,687,402 | 60,687,422 | TTCACCCACCTTGTACCTCAG |
| TDF | 60,666,866 | 60,666,884 | CGCCGTCTCCTGTCCTAAG |
| TDP | 60,666,829 | 60,666,856 | TACAGAGTCCATCTTTCCTCCCACAGGG |
| TDR | 60,666,786 | 60,666,807 | GAGCAGGGAAGAGTCTGAATGG |
PCR Primers
| Primer | Sequence |
| SU | AAGTCATGGTTGCCATAGCC |
| SD | GAGGCATCCTTAGAACTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 440 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11386B-E12 | VGB6 | C57BL/6NTac | |
| 11386B-C4 | VGB6 | C57BL/6NTac | |
| 11386C-G11 | VGB6 | C57BL/6NTac | |
