| Gene | Gcc1 |
| Chromosome | 6 |
| Deletion Start | 28,421,313 |
| Deletion End | 28,417,957 |
| Deletion Size | 3,357 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,420,642 | 28,420,663 | GCAAGAGCAGATAGCAGAAACC |
| TUP | 28,420,616 | 28,420,639 | AGCCCGCCTCATTACACAGCAGCA |
| TUR | 28,420,567 | 28,420,585 | AAGCTCGCGCAGCATCAAG |
| TDF | 28,418,411 | 28,418,432 | CCGACACAGCCTCCTCAGATAG |
| TDP | 28,418,389 | 28,418,410 | CTTGACCCAGGCCCTGCAGCTT |
| TDR | 28,418,348 | 28,418,371 | TGCTCAGCATAAAGAAAGAAGGTG |
PCR Primers
| Primer | Sequence |
| SU | TGCTAGGTCAGCGTCACTC |
| SD | CACTACGAGATGCTGTCTCA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 293 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11391A-B11 | VGB6 | C57BL/6NTac | |
