| Gene | Zfp526 |
| Chromosome | 7 |
| Deletion Start | 25,224,244 |
| Deletion End | 25,226,342 |
| Deletion Size | 2,117 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,224,394 | 25,224,413 | CAGGAGAGCTGGCAGAGATG |
| TUP | 25,224,415 | 25,224,442 | CGACAGCAGTGACTGAGATGACCTCTGG |
| TUR | 25,224,462 | 25,224,481 | TGGTGGTGCTGGAAGAAGAG |
| TDF | 25,226,321 | 25,226,340 | GCAGTTGGATACGGCCTTTG |
| TDP | 25,226,342 | 25,226,364 | CTGACACGGCGCAAGAGCAATGA |
| TDR | 25,226,378 | 25,226,398 | GCCCAGACACAAGCTGGTAAG |
PCR Primers
| Primer | Sequence |
| SU | GGGAGAGGAGGTAAAGTGGG |
| SD | CCCAAACATCCCAAGTTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 508 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 499 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11392A-D9 | VGB6 | C57BL/6NTac | |
