| Gene | Klrg2 |
| Chromosome | 6 |
| Deletion Start | 38,637,063 |
| Deletion End | 38,627,585 |
| Deletion Size | 9,479 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,636,220 | 38,636,240 | CCCGGAAACTTTCCTTCCTTC |
| TUP | 38,636,195 | 38,636,219 | TTAGTGTGCGCAGCATCTTGGAGAA |
| TUR | 38,636,172 | 38,636,194 | CCTCACCTTACCCAGAGTTCTAC |
| TDF | 38,628,270 | 38,628,292 | TGAGCGGATAACTTCCTGGAAAG |
| TDP | 38,628,245 | 38,628,266 | TGCTGGAATCCTGCAGAAGGCC |
| TDR | 38,628,194 | 38,628,214 | GGGAAAAGCAGGCAGTTACTC |
PCR Primers
| Primer | Sequence |
| SU | TGTTAGTTGACAACGTGAACG |
| SD | GTATGTCAACAGTGACAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 283 bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 300 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11393A-C6 | VGB6 | C57BL/6NTac | |
| 11393A-D7 | VGB6 | C57BL/6NTac | |
| 11393A-E4 | VGB6 | C57BL/6NTac | |
| 11393A-E8 | VGB6 | C57BL/6NTac | |
| 11393A-F6 | VGB6 | C57BL/6NTac | |
