| Gene | Insm2 |
| Chromosome | 12 |
| Deletion Start | 55,599,476 |
| Deletion End | 55,600,966 |
| Deletion Size | 1,491 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,600,118 | 55,600,135 | TTCGCTCTGGCTCAGCAC |
| TUP | 55,600,144 | 55,600,163 | CCGCATCGTGCGCGTCGAGT |
| TUR | 55,600,175 | 55,600,196 | AGCTGAAGACCTTGTCGCACTC |
| TDF | 55,600,557 | 55,600,579 | AGTTCCGTCGCCAAGCCTATCTG |
| TDP | 55,600,582 | 55,600,604 | CAAGCACCTGGGCACCCATGAGA |
| TDR | 55,600,623 | 55,600,641 | GAGCCAAAACCCGGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | GGTGGCTCTGGCTCCCTAG |
| SD | CTGTCAGCGTTGACCGTGA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 405 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 304 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11401A-E6 | VGB6 | C57BL/6NTac | |
| 11401A-F1 | VGB6 | C57BL/6NTac | |
