11409   Ddx28     Status: ES cell colonies screened / QC positives

GeneDdx28
Chromosome8
Deletion Start106,011,421
Deletion End106,009,778
Deletion Size1,644
Genome Build38
MGI IDMGI:1919236
NCBI Gene ID71986
BAC ID315H10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF106,010,820106,010,838TGGACTCCCGCAGTTTCAC
TUP106,010,789106,010,813TCGAGGCTTGGTTCTGGTACCTTCC
TUR106,010,767106,010,787GCACCTGTTCGGCTAGTTCTC
TDF106,010,225106,010,243GGAACCCTCGGGAACTGTC
TDP106,010,201106,010,223TGGTATTCTGCAACAGCGCCAGC
TDR106,010,137106,010,155GCCCTTGCAGCCTTAGATG

PCR Primers

PrimerSequence
SUCTACTGCGGACTCCCATTCC
SDAATCATGACATAGTGTAAAGTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev364 bp-++
TUFTUR72 bp++-
TDFTDR107 bp++-
NeoFwdSD273 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11409A-C7VGB6C57BL/6NTac
11409A-E5VGB6C57BL/6NTac
11409A-E10VGB6C57BL/6NTac
11409A-F5VGB6C57BL/6NTac