| Gene | Ddx28 |
| Chromosome | 8 |
| Deletion Start | 106,011,421 |
| Deletion End | 106,009,778 |
| Deletion Size | 1,644 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,010,820 | 106,010,838 | TGGACTCCCGCAGTTTCAC |
| TUP | 106,010,789 | 106,010,813 | TCGAGGCTTGGTTCTGGTACCTTCC |
| TUR | 106,010,767 | 106,010,787 | GCACCTGTTCGGCTAGTTCTC |
| TDF | 106,010,225 | 106,010,243 | GGAACCCTCGGGAACTGTC |
| TDP | 106,010,201 | 106,010,223 | TGGTATTCTGCAACAGCGCCAGC |
| TDR | 106,010,137 | 106,010,155 | GCCCTTGCAGCCTTAGATG |
PCR Primers
| Primer | Sequence |
| SU | CTACTGCGGACTCCCATTCC |
| SD | AATCATGACATAGTGTAAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 364 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 273 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11409A-C7 | VGB6 | C57BL/6NTac | |
| 11409A-E5 | VGB6 | C57BL/6NTac | |
| 11409A-E10 | VGB6 | C57BL/6NTac | |
| 11409A-F5 | VGB6 | C57BL/6NTac | |
