| Gene | Trem3 |
| Chromosome | 17 |
| Deletion Start | 48,247,852 |
| Deletion End | 48,258,547 |
| Deletion Size | 10,895 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,248,559 | 48,248,578 | TGGGCCACCAGACATACAAC |
| TUP | 48,248,588 | 48,248,616 | TAGAGAACTGGGAATAGTGGGACATGCCT |
| TUR | 48,248,617 | 48,248,636 | CTCCCGACTGCTAGGATGAC |
| TDF | 48,257,759 | 48,257,777 | GCAAGGCAGGTAGCAGGTC |
| TDP | 48,257,778 | 48,257,801 | CAACCACAGAGCCCTGAAGGCAGG |
| TDR | 48,257,832 | 48,257,855 | GCAAGTGTGAGCACTCCAAGAATG |
PCR Primers
| Primer | Sequence |
| SU | GATTGTGGCATCAAAGGAGG |
| SD | GAGATAGGTTACATCATATAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11412A-E5 | VGB6 | C57BL/6NTac | |
| 11412B-B12 | VGB6 | C57BL/6NTac | |
| 11412B-H2 | VGB6 | C57BL/6NTac | |
