| Gene | Nes |
| Chromosome | 3 |
| Deletion Start | 87,971,189 |
| Deletion End | 87,980,040 |
| Deletion Size | 8,852 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,971,926 | 87,971,947 | GCGCTGGAACAGAGATTGGAAG |
| TUP | 87,971,957 | 87,971,976 | AGGACCGGCTGCAGGCCACT |
| TUR | 87,972,048 | 87,972,070 | TGAAGGTCTCTTGCCATCCTAAG |
| TDF | 87,979,081 | 87,979,103 | CTCTCCCTGACTCTACTCCCTTG |
| TDP | 87,979,106 | 87,979,131 | CCTCTACCTGAAGTCTCCTGCCTCCC |
| TDR | 87,979,132 | 87,979,152 | CTCCAGCTTGCTCCCACTTTG |
PCR Primers
| Primer | Sequence |
| SU | TCGCTTCAGCTCGCTGCTG |
| SD | TTGAACTATAGGCTCAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 261 bp | - | + | + |
| TUF | TUR | 145 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 261 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11414A-B8 | VGB6 | C57BL/6NTac | |
| 11414A-E3 | VGB6 | C57BL/6NTac | |
| 11414A-F1 | VGB6 | C57BL/6NTac | |
