| Gene | Zfp575 |
| Chromosome | 7 |
| Deletion Start | 24,586,726 |
| Deletion End | 24,585,483 |
| Deletion Size | 1,244 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,586,672 | 24,586,691 | GGAACCTAGTCCCACCTGTC |
| TUP | 24,586,645 | 24,586,668 | ACCCGGAGACCAAAGGTGAGAGCA |
| TUR | 24,586,595 | 24,586,612 | GTTCTCCTGGGCTGTCAC |
| TDF | 24,585,589 | 24,585,606 | TACTGGCAGCCACCGATG |
| TDP | 24,585,564 | 24,585,586 | CCAGCTGCAATCAGGCCTTTGGC |
| TDR | 24,585,535 | 24,585,554 | CGTTGGTGAACCAGCAGGAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTAGTGTGTACTTAGGCTC |
| SD | TTCCTGCAGAACCAATAATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 326 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 300 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11427A-A5 | VGB6 | C57BL/6NTac | |
| 11427A-F12 | VGB6 | C57BL/6NTac | |
| 11427A-G5 | VGB6 | C57BL/6NTac | |
| 11427A-G4 | VGB6 | C57BL/6NTac | |
| 11427A-G7 | VGB6 | C57BL/6NTac | |
| 11427A-G1 | VGB6 | C57BL/6NTac | |
