| Gene | Kiss1 |
| Chromosome | 1 |
| Deletion Start | 133,327,253 |
| Deletion End | 133,329,662 |
| Deletion Size | 5,106 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,572,557 | 55,572,577 | ACACCCACAGTTGAGCTACAG |
| TUP | 55,572,534 | 55,572,555 | TGCGCCTGGGCAGATAAACCGT |
| TUR | 55,572,507 | 55,572,531 | GCAAATGACATCTAGCGAAGGAAAC |
| TDF | 55,568,760 | 55,568,779 | TTAGCTGGCCCTACCGTTTC |
| TDP | 55,568,737 | 55,568,758 | TGCGCAAGTACGGCTCTGACAA |
| TDR | 55,568,689 | 55,568,710 | TTACCACCTTTCCAGACAGACC |
PCR Primers
| Primer | Sequence |
| SU | CTCCTTGGAGCAGCAAGAAC |
| SD | CTGTTCAATCTGTACAGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 77754176 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 77761986 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11430E-F12 | VGB6 | C57BL/6NTac | |
| 11430G-A6 | VGB6 | C57BL/6NTac | |
| 11430G-C5 | VGB6 | C57BL/6NTac | |
| 11430G-G4 | VGB6 | C57BL/6NTac | |
