| Gene | Hsd17b6 |
| Chromosome | 10 |
| Deletion Start | 127,998,007 |
| Deletion End | 127,991,182 |
| Deletion Size | 6,826 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,997,139 | 127,997,160 | TGGAGTTTGGTCACCGTAGAAG |
| TUP | 127,997,109 | 127,997,133 | ACTCTCCCAGCAGCTACCAATTGCC |
| TUR | 127,997,061 | 127,997,083 | GGAAAGAAGAGGTGAGCATGAAG |
| TDF | 127,991,479 | 127,991,496 | GCTGGCTGACCACCTTTC |
| TDP | 127,991,454 | 127,991,478 | TCTGCTTCTTCAGTTTGCAGCACCA |
| TDR | 127,991,416 | 127,991,437 | GCTCAGGTTCCTGCTACACTTC |
PCR Primers
| Primer | Sequence |
| SU | GAGACAAAGCTTGGAATGTTTC |
| SD | CTCCTCAGGCACCAACACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11433A-G12 | VGB6 | C57BL/6NTac | |
| 11433A-E8 | VGB6 | C57BL/6NTac | |
| 11433A-F4 | VGB6 | C57BL/6NTac | |
| 11433A-H3 | VGB6 | C57BL/6NTac | |
