| Gene | Wdr5b |
| Chromosome | 16 |
| Deletion Start | 36,041,495 |
| Deletion End | 36,042,500 |
| Deletion Size | 1,006 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,041,572 | 36,041,591 | CGAGGAAGAGCCGCAGAAAC |
| TUP | 36,041,592 | 36,041,621 | CCAACTATGCTCTCAGACTTACTCTTGCGG |
| TUR | 36,041,624 | 36,041,643 | GATGATATGGCCGCCGAGTG |
| TDF | 36,042,059 | 36,042,081 | GGGAGCTATGATGGGCTTTGTAG |
| TDP | 36,042,082 | 36,042,106 | AATTTGGGATGCTGCGTCAGGCCAG |
| TDR | 36,042,117 | 36,042,137 | GGTTGCCTTCATCAGCAAGTG |
PCR Primers
| Primer | Sequence |
| SU | GATCACTGTGATCCGTGATG |
| SD | TGAGCACAGGAGATTCCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 301 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 278 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11435A-B5 | VGB6 | C57BL/6NTac | |
| 11435A-D9 | VGB6 | C57BL/6NTac | |
| 11435A-D10 | VGB6 | C57BL/6NTac | |
