| Gene | Rbm12b |
| Chromosome | 4 |
| Deletion Start | 12,093,146 |
| Deletion End | 12,095,672 |
| Deletion Size | 2,527 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,094,929 | 12,094,950 | GGCAGTGTCATGAGAAAGATCA |
| TUP | 12,094,952 | 12,094,974 | AGGCAGCTAAGAAGCCCTTGGGA |
| TUR | 12,094,996 | 12,095,016 | GATCTCCTGAAGTGCTCCTGG |
| TDF | 12,095,107 | 12,095,129 | GCAAGCTTCTGATGAGGACTTCA |
| TDP | 12,095,152 | 12,095,181 | ATATCCCACTGATGAGGACTTTAGGCGGGT |
| TDR | 12,095,246 | 12,095,266 | GGTCCAAAACTCCTCTCCAGA |
PCR Primers
| Primer | Sequence |
| SU | ATGCTGAAGAAGGCAGGAAC |
| SD | CAATGAACCGAATGAATTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 160 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11439A-E5 | VGB6 | C57BL/6NTac | |
| 11439A-H5 | VGB6 | C57BL/6NTac | |
| 11439A-E10 | VGB6 | C57BL/6NTac | |
| 11439A-G5 | VGB6 | C57BL/6NTac | |
