| Gene | Rab9b |
| Chromosome | X |
| Deletion Start | 136,861,646 |
| Deletion End | 136,860,991 |
| Deletion Size | 656 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,861,422 | 136,861,444 | TCAAGAGCCTTAGAACACCATTC |
| TUP | 136,861,391 | 136,861,413 | AGCCGACTGCTGCCTGCTAACCT |
| TUR | 136,861,363 | 136,861,382 | TCAAAGCTCTGCCGGTCATC |
| TDF | 136,861,232 | 136,861,251 | AACGACTGAGGAGGCACAAG |
| TDP | 136,861,204 | 136,861,231 | CCTGGTGCATGGAGAATGGAAATTACCC |
| TDR | 136,861,147 | 136,861,167 | GCTTCTTCAAAGGCCACTGTC |
PCR Primers
| Primer | Sequence |
| SU | TCATGGCATTCAGCCTTTCC |
| SD | AATACACTTGGCTCAATAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 396 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 407 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11442A-F3 | VGB6 | C57BL/6NTac | |
| 11442A-G12 | VGB6 | C57BL/6NTac | |
| 11442A-H1 | VGB6 | C57BL/6NTac | |
| 11442A-H7 | VGB6 | C57BL/6NTac | |
| 11442A-H12 | VGB6 | C57BL/6NTac | |
