| Gene | Thsd1 |
| Chromosome | 8 |
| Deletion Start | 22,238,543 |
| Deletion End | 22,259,672 |
| Deletion Size | 21,130 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,238,577 | 22,238,597 | TGGTGGTGCTCTGTGACTATG |
| TUP | 22,238,599 | 22,238,626 | TGAGTATGGTGGAGAACACATGAAGCCA |
| TUR | 22,238,644 | 22,238,662 | TCACGAAGCATGGCCTTTG |
| TDF | 22,259,019 | 22,259,039 | AGATGGCTCATCGGAGAGGTG |
| TDP | 22,259,044 | 22,259,069 | TCCAGAAGTTCCCTCTTCAGGAGGAC |
| TDR | 22,259,083 | 22,259,103 | AGGGCGAGACTGCTTGGTTTC |
PCR Primers
| Primer | Sequence |
| SU | TTAACCACAGCCAGCCAGG |
| SD | AGTAACAAGCCAATAGAAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 331 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 308 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11443A-B8 | VGB6 | C57BL/6NTac | |
| 11443B-B7 | VGB6 | C57BL/6NTac | |
| 11443B-C1 | VGB6 | C57BL/6NTac | |
| 11443B-C9 | VGB6 | C57BL/6NTac | |
