| Gene | Nkg7 |
| Chromosome | 7 |
| Deletion Start | 43,437,307 |
| Deletion End | 43,438,098 |
| Deletion Size | 792 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,437,394 | 43,437,411 | GCCACAGGTCCTCACTTC |
| TUP | 43,437,414 | 43,437,433 | TGCCCACTCTGGCCTCTGGC |
| TUR | 43,437,434 | 43,437,456 | TACTTGAGTCTCTTGGCTTGTTG |
| TDF | 43,437,807 | 43,437,829 | ACCAGTATGAGATGGAGCCAGAC |
| TDP | 43,437,838 | 43,437,866 | CTCAAGTCCAGACATTCTTCTCCTGGTCC |
| TDR | 43,437,887 | 43,437,910 | CCTGCAAAGAGGAAGAGGATGAAG |
PCR Primers
| Primer | Sequence |
| SU | GAAGGTGTGAGAAGAATCAG |
| SD | TGGCTCGGTTTATGTCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11445A-A9 | VGB6 | C57BL/6NTac | |
| 11445A-C2 | VGB6 | C57BL/6NTac | |
| 11445A-D4 | VGB6 | C57BL/6NTac | |
| 11445A-D7 | VGB6 | C57BL/6NTac | |
| 11445A-D12 | VGB6 | C57BL/6NTac | |
| 11445A-G10 | VGB6 | C57BL/6NTac | |
