| Gene | Prkcdbp |
| Chromosome | 7 |
| Deletion Start | 105,482,156 |
| Deletion End | 105,480,788 |
| Deletion Size | 1,369 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,481,675 | 105,481,694 | CCGTCCGCATCTCCACATTC |
| TUP | 105,481,655 | 105,481,673 | TGACCCAGCGTGCTGCCCT |
| TUR | 105,481,586 | 105,481,608 | GGGAGTGAGAAAGGGATAAGGTC |
| TDF | 105,481,086 | 105,481,105 | GAAGAGGAGCCAGTGGAGTC |
| TDP | 105,481,056 | 105,481,075 | CGGCTGCGCCGCACTGGTTT |
| TDR | 105,480,999 | 105,481,017 | GTGGGCTGTGCTGCTTTAC |
PCR Primers
| Primer | Sequence |
| SU | CAGGACTGTCTTACTCGCAG |
| SD | TGGTGAACCTAAATTAGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 337 bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 261 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11448A-A5 | VGB6 | C57BL/6NTac | |
| 11448A-A12 | VGB6 | C57BL/6NTac | |
| 11448A-C3 | VGB6 | C57BL/6NTac | |
| 11448A-D8 | VGB6 | C57BL/6NTac | |
| 11448A-C6 | VGB6 | C57BL/6NTac | |
